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A Precision Flow-Controlled Rb-82 Generator for Bolus or Constant-Infusion Studies of the Heart and Brain

Donner Laboratory and Lawrence Berkeley Laboratory, University of California, Berkeley, California

Correspondence: For reprints contact: Yukio Yano, Rm. 224, Donner Laboratory, University of California, Berkeley, CA 94720.

ABSTRACT

A precision flow-controlled rubidium-82 generator has been constructed to deliver 76-sec Rb-82 at either fast or slow flow rates for bolus or constant-infusion studies. A stepping motor drive is interfaced to a microprocessor for pulsed control of flow rate to deliver the saline eluant solution from a large-volume (150 ml) machined pumping syringe through an alumina column that retains the 25-day Sr-82 parent. The generator system delivers 70–90% of the maximum Rb-82 activity in a 20–25 ml bolus elution of 2% NaCl.The Sr-82/Sr-85 breakthrough is 10^–7–10^–6. Both yield and breakthrough are functions of column length and flow rate. Six separate Sr-82 loadings of the generator were evaluated over a period of nearly 2 yr in studies of myocardial blood perfusion and permeability changes in the blood-brain barrier. Sterility and apyrogenicity of the Rb-82 eluate were maintained during multiple elutions and long-term use of 3–4 mo for each generator loading.