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In Vitro Assessment of Tc-99m Labeled Bovine Thrombin and Streptokinase-Activated Human Plasmin: Concise Communication

Martin Luther King Jr. General Hospital and Charles R. Drew Postgraduate Medicine School, Los Angeles, California

Correspondence: For reprints contact: Dennis W. Wong, Pharm. D., Dept. of Nuclear Medicine, Martin Luther King, Jr. General Hospital, 12021 S. Wilmington Ave., Los Angeles, CA 90059.

ABSTRACT

Bovine thrombin and streptokinase-activated human plasmin have been labeled with Tc-99m using stannous reduction of pertechnetate under physiological conditions (pH 7.4). The binding efficiency of radiotechnetium to these enzymes is greater than 94%, with less than 5% of reduced but unbound Tc-99m (Sn) complex as assayed by ascending paper radiochromatography using ITLC silica gel plate. Free or unbound pertechnetate is less than 1%. In vitro enzymatic analyses of the Tc-99m-labeled enzymes demonstrate no evidence of protein denaturation or significant loss of enzymatic activity after labeling. Both labeled enzymes are biochemically active in vitro with their respective substrates.