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A Transferrin-Mediated Uptake of Gallium-67 by EMT-6 Sarcoma. I. Studies in Tissue Culture

Veterans Administration Hospital and the University of Washington Medical School, Seattle, Washington

Correspondence: For reprints contact: Steven Larson, Nuclear Medicine Section (115), Veterans Administration Hospital, 4435 Beacon Ave. So., Seattle, WA. 98108.

ABSTRACT

We have studied the in vitro uptake of gallium-67 by exponentially growing EMT-6 sarcoma cells in long-term tissue culture. In this system, the addition of transferrin to the medium was required before an appreciable cellular uptake of Ga-67 occurred. The transferrin effect was complex, with an initial stimulation to a peak cell-to-medium ratio of 8–10:1 at low concentrations of transferrin (0.2 mg/ml), followed by a gradual decline in uptake as transferrin in the medium was increased further. EMT-6 tumor-cell uptake of Ga-67 was probably mediated by a specific cellular receptor for transferrin. Scatchard analysis of the EMT-6 cellular binding of human transferrin labeled with iodine-125 indicated a cellular receptor with affinity for transferrin of 5 x 10⁶ l/mole and abundance of 500,000 receptors per cell. Over the experimental range of transferrin concentration in the medium, the observed uptake of Ga-67 was closely correlated with the degree of formation of Ga-67-labeled transferrin and the fraction of transferrin bound to the cellular receptor (N = 69, r = 0.86, p < 0.0001).

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