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Scintillation-Proximity Assay of Antigen—Antibody Binding Kinetics: Concise Communication

City College of New York, New York, New York
Montefiore Hospital and Medical Center, Bronx, New York

Correspondence: For reprints contact: Hiram E. Hart, Dept. of Physics, The City College. The City University of NY, New York, NY 10031.

ABSTRACT

Tritiated latex particles and scintillant latex particles have been covalently coated with an antigen (human albumin) and both types of particles are then combined in dilute aqueous suspensions. The formation of antibody-induced dimer and higher-order aggregation resulted in scintillations that could be measured in a standard liquid-scintillation counter, energy-gated for H-3. Rabbit anti-human anti-serum was easily detected at a titer of 1:10⁶. Human albumin, in the inhibition mode, was also easily detected at 0.1 ng/ml. Since the samples are nondestructively assayed, it was possible to examine the kinetics of aggregation under a variety of conditions.