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Semi-Automated T₃ Uptake Test That Uses Magnetic Albumin Microparticles

3M Company, St. Paul, Minnesota

Correspondence: ¹ For reprints contact: D. S. Ithakissios, Medical Products Div., 3M Co., 3M Ctr.—230-3, St. Paul, MN 55101.

ABSTRACT

We describe a T₃ uptake test that uses magnetic albumin microparticles to separate free from bound [¹²⁵I] T₃. The test uses 0.1 ml serum in a total assay volume of 1.1 ml, and is performed in barbital buffer of pH 7.6, ionic strength 0.2. The accuracy of the test is not affected by the following ranges: volume, 1–3 ml; incubation time, 15–120 min; and incubation temperature, 4–35°C. We describe a magnetic separator device that allows simultaneous handling of 100 assay tubes with minimum manipulation. The intra-assay and interassay coefficients of variance were 2.87% and 5.20%, respectively.