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Liquid Scintillation Vial for Radiometric Assay of Lymphocyte Carbohydrate Metabolism in Response to Mitogens

The Johns Hopkins Medical Institutions, Baltimore, Maryland

Correspondence: For reprints contact: Ngo M. Tran, Dept. of Radiology, University of California, Irvine Medical Ctr., 101 City Dr. S., Orange, CA 92668.

ABSTRACT

We have demonstrated that mitogens—i.e., PHA and Con.A—stimulate lymphocyte carbohydrate metabolism using a liquid-scintillation vial with conventional liquid-scintillation detectors. The results showed that this enclosed system can be useful for development of rapid in vitro tests of lymphocytes immune responsiveness, as well as for radiometric detection of bacterial growth in various gaseous atmospheres.