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Indium-111-Labeled Cellular Blood Components: Mechanism of Labeling and Intracellular Location in Human Neutrophils

Hammersmith Hospital, Northwick Park Hospital, and Washington University, St. Louis, Missouri
Royal Postgraduate Medical School, London, United Kingdom

Correspondence: For reprints contact: Mathew L. Thakur, Yale University School of Medicine, Department of Diagnostic Radiology, Nuclear Medicine, 333 Cedar Street, New Haven, CT 06510.

ABSTRACT

Human neutrophils were labeled with In-111 oxine by incubation at room temperature, and the fate of the oxine and the intracellular location of the In-111 were determined. Neutral (1:3) In-111 oxine complex diffuses rapidly across the cell membrane and then dissociates. Some of the oxine leaves the cell and the In-111 binds intracellularly. After short periods of incubation the label is distributed mostly to four soluble components, a small proportion of which had a distribution similar to that of specific and azurophil granules. After longer incubation periods there was relatively less radioactivity with the soluble components and probably more attached to the particulate material. A small peak of radioactivity also appeared in the region of DNA distribution, but no confirmation could be obtained for the association of the In-111 radioactivity with DNA.

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