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A Comparative Evaluation of Techniques for Rapid and Efficient In Vivo Labeling of Red Cells with [^99mTc] Pertechnetate

Armed Forces Radiobiology Research Institute, Bethesda, Maryland
Johns Hopkins Hospital, Baltimore, Maryland

Correspondence: For reprints contact: Lt. Hamilton, Armed Forces Radiobiology Research Institute, Bethesda, MD 20014.

ABSTRACT

Red blood cells (RBCs) labeled in vivo with ^99mTcO₄^– have recently been recommended for blood-pool imaging, but the optimum conditions for in vivo labeling of RBCs have not been clearly defined. We therefore evaluated several stannous-ion preparations and stannous-ion concentrations to determine which provided the best labeling. The effect of the time interval between the Sn(II) and ^99mTcO₄^– injections and the effect of carrier technetium on labeling efficiency were also studied. Maximal in vivo labeling efficiency was obtained using an intravenous dose of 10 µg Sn(II)/kg followed 5–30 min later by an injection of ^99mTcO₄^–. Neither the chelated form of stannous ion used in these studies nor the amount of carrier present had a significant effect on labeling efficiency. The biologic half-time of Tc-99m RBCs labeled in vivo was similar to that of Tc-99m RBCs labeled in vitro. In vivo labeling is a rapid and efficient method for the preparation of Tc-99m RBCs.