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Labeling Human Lymphocytes with Mercury-197

McGill University, Montreal, Quebec
University of Arkansas for Medical Sciences, Little Rock, Arkansas

Correspondence: For reprints contact: Gopal B. Saha, Div. of Nuclear Medicine, University of Arkansas Medical Center, Little Rock, AR 72201.

ABSTRACT

A simple method for labeling human lymphocytes with Hg-197 is described. Lymphocytes were isolated from human blood and purified by the Ficoll-Hypaque method. Labeling was carried out by incubating viable cells suspended in saline with Hg-197 for different time intervals and at different temperatures. The labeling yield varied from 48% for 1 million cells to about 70% for 10 million cells after incubation with 2 µCi of Hg-197 for 2 hr at 37°C. The temperature and incubation period beyond 1 hr had no appreciable effect on the labeling yield. The yield increased by a factor of 1.5 when the cells were stimulated by phytohemagglutinin. Subcellular distribution studies showed that 60% of the tracer localized in the nucleus and cell membrane, while the remaining portion concentrated in the mitochondrial and microsomal fractions.