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Radioiodinated Plasminogen: An Imaging Agent for Pre-Existing Thrombi

Mallinckrodt Institute of Radiology, St. Louis, Missouri

Correspondence: For reprints contact: Michael J. Welch, Mallinckrodt Institute of Radiology, 510 S. Kingshighway Blvd., St. Louis, MO 63110.

ABSTRACT

We have reinvestigated radioiodinated plasminogen as an agent for localizing preformed thrombi. Canine plasminogen was isolated from fresh plasma by the affinity chromatography technique on a lysine-sepharose 4B column and tagged with I-123 or I-131, at less than one iodine atom per molecule of enzyme, by the conventional ICl method. When injected into dogs more than 2 days after thrombus induction, radioiodinated plasminogen produced thrombus-to-blood activity ratios of 7.8 ± 2.4. Thrombi as old as 6 days can be visualized in 80% of the cases. Both the weight of the thrombus and the thrombus-to-blood ratio are more variable for 1-day-old thrombi; this may be associated with plasminogen release accompanying thrombus retraction. The results suggest that radioiodinated plasminogen has potential as an imaging agent for pre-existing thrombi.

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