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Toadfish Serum as a Binder for In Vitro Assay of Vitamin B₁₂

Johns Hopkins Medical Institutions, Baltimore
University of Maryland, Solomons, Maryland

Correspondence: For reprints contact: Henry N. Wagner, Jr., 615 N. Wolfe St., Baltimore, MD 21205.

ABSTRACT

Serum from the oyster toadfish, Opsanus tau, has a binding capacity for cyanocobalamin 1,000 times greater than that of human serum. The binding follows the principle of isotope dilution in the physiologic range of vitamin B₁₂ present in human serum. Under proper conditions of storage, this binder is stable for at least 1 year. Standard reagents and techniques used in other vitamin B₁₂ competitive binding assays can be used with the toadfish serum binder. Toadfish serum offers potential advantages over intrinsic factor and human serum, the most commonly used binders in vitamin B₁₂ assays.