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In Vitro Studies of Leukocyte Labeling with Technetium-99m

Kanematsu Memorial Institute, Sydney Hospital, Sydney, Australia

Correspondence: For reprints contact: T. Uchida, Medical Research Dept., Kanematsu Memorial Institute, Sydney Hospital, Sydney, N.S.W. 2000, Australia.

ABSTRACT

A method for labeling leukocytes in vitro with ^99mTc is described. Separated leukocytes are incubated with ^99mTc, followed by reduction with stannous chloride and washing with acid citrate dextrose solution. Maximum labeling occurs after at least 5 min incubation with pertechnetate, followed by at least 10 min incubation with stannous chloride. Labeling is similar at room temperature and at 37°C. The labeled leukocytes are viable, and reutilization of label does not occur in vitro. In acid conditions (pH 5.2), the elution of ^99mTc from leukocytes is comparable with that of ³²P-diisopropylfluorophosphate, but ^99mTc elution is greater at pH 7.2–7.4. Neutrophils label more heavily with ^99mTc than do monocytes, lymphocytes, erythrocytes, or platelets.