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A Simple and Rapid Radioimmunoassay of Triiodothyronine in Unextracted Serum

Veterans Administration Hospital, Jefferson Barracks and Washington University, St. Louis, Missouri

Correspondence: For reprints contact: Bhartur N. Premachandra, V.A. Hospital, Jefferson Barracks and Washington University, St. Louis, Mo. 63125.

ABSTRACT

A simple, accurate, and rapid procedure for radioimmunoassay (RIA) of triiodothyronine (T₃) is described. Serum is denatured by sodium trichloroacetate and the T₃ released is allowed to react with ¹²⁵I-T₃-labeled T₃ anti-serum. The displaced ¹²⁵I-T₃ is rapidly taken up by an anionic-resin sponge, and this uptake is linearly related to serum T₃ concentration. The entire assay procedure was completed in the same tube in about 1 hr. The T₃ antibody was specific, and T₄-to-T₃ conversion in the assay did not occur. Recovery of exogenously added T₃ was virtually complete. The mean T₃ concentration in 92 euthyroid subjects (142 ng/100 ml) is comparable to the values described in a number of radioimmunoassays for T₃.