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Vesicle Interactions with Antibody and Peptide Hormone: Role of Vesicle Composition

June K. Dunnick^*, Rodney S. Badger, Yosuke Takeda and Joseph P. Kriss

Stanford University Medical Center, Stanford, California

Correspondence: For reprints contact: Joseph P. Kriss. Div. of Nuclear Medicine, Dept. of Radiology, Stanford University Medical Center, Stanford, CA 94305.

ABSTRACT

Artificial lipid vesicles (artificial membranes) were shown to bind human ¹²⁵I-antithyroglobulin (anti-Tg) and human ¹²⁵I-thyrotropin. Vesicles made with gangliosides bound more antibody and hormone than vesicles lacking them. These gangliosides contained a variety of carbohydrates including glucose, galactose, N-acetyl-galactosamine, and sialic acid. The in vivo stability of antibody-vesicle complexes was a function of vesicle composition: vesicles were most stable when formed from phosphatidylcholine, cholesterol, and gangliosides. Anti-Tg-vesicle complexes bind to thyroglobulin, indicating that at least some of the antibody associated with the vesicle still retains ability to bind to its specific antigen. The addition of a specific antibody or hormone to artificial lipid vesicles may serve as a mechanism to confer specificity to the vesicle in vivo.

FOOTNOTES

^* Present address: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20014.

Present address: Central Clinical Radioisotope Div., Kyoto University, Kyoto, Japan.