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Technetium-99m Labeling of Human Monocytes for Chemotactic Studies

Michael Reese Hospital and Medical Center, Pritzker School of Medicine, University of Chicago, Chicago, Illinois

Correspondence: For reprints contact: R. R. Kretschner, Div. of Immunology, Dept. of Pediatrics, Michael Reese Medical Center, 29th St. and Ellis Ave., Chicago, IL 60616.

ABSTRACT

Although the current use of ⁵¹Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium-99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte-derived chemotactic factor. The chemotactic properties of ^99mTc-labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.