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Labeling and Testing of ^99mTc-Streptokinase for the Diagnosis of Deep Vein Thrombosis

University of Lund and Centrallasarettet, Östersund, Sweden

Correspondence: For reprints contact: Bertil R. R. Persson, Dept. of Radiation Physics, Lasarettet, S-221 85 Lund, Sweden.

ABSTRACT

A detailed study has been made of the method for labeling streptokinase with ^99mTc in order to get a radioactive indicator for rapid scintigraphic visualization of thrombi and emboli. The best method found for preparing ^99mTc-streptokinase was by reducing ^99mTc-pertechnetate with 2 µmole SnCl₂ at pH 0.7 and then adding 50,000–75,000 IU of streptokinase at a final pH of about 2. After 1 hr of equilibration the labeling efficiency was 75–80% as determined by the method of gel chromatography column scanning. The dynamic behavior and distribution of ^99mTc-streptokinase in different organs was studied in rabbits where a high uptake was found in liver. In a clinical investigation using ^99mTc-streptokinase prepared according to this work, however, the liver uptake in man was only 10–20%.

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