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Autoradiography of Live and Dead Mammalian Cells with ^99mTc-Tetracycline

New England Medical Center and Tufts University School of Medicine, Boston, Massachusetts

Correspondence: For reprints contact: Mrinal K. Dewanjee, Dept. of Radiology, Div. of Nuclear Medicine, Proger 4, Tufts Medical School and New England Medical Center, Boston, Mass. 02111.

ABSTRACT

Considering the diagnostic significance of a few ^99mTc-chelates in the diagnosis of myocardial infarct, we have recently developed a necrosis model in tissue culture obtained from mammalian cells, where the dead cells showed enhanced binding of ^99mTc-tetracycline like the necrotic cells of infarcted myocardium. Technical details of the principles of labeling, fixing, washing and drying, dipping in emulsion, exposure, development and staining of mammalian cells have been described. Considering the short half-life of ^99mTc-radioisotope, the recently available techniques have been modified to make autoradiography possible in a reasonably short period of time. Technetium-99m in different physical and chemical states is playing a very important role in a variety of diagnostic procedures. The autoradiography of ^99mTc-labeled cells and tissue will shed further light on the mechanism of cellular uptake and subcellular distribution in normal and pathologic states. Presently no information is available regarding autoradiography with ^99mTc-chelates of mammalian cells obtained from tissue culture.