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Survival and Sequestration of ⁵¹Cr- and ^99mTcO₄-Labeled Platelets

Kyoto University, Kyoto, Japan

Correspondence: For reprints contact: Tatsumi Uchida, Kanematsu Memorial Institute, Sydney Hospital, Sydney NSW, 2000, Australia.

ABSTRACT

A new method by which platelets can be labeled with ^99mTc and ⁵¹Cr has been developed by the authors and is presented here. Platelets separated by Aster's acid-citrate method were incubated with ^99mTc and ⁵¹Cr followed by reduction with stannous chloride (SnCl₂ · 2H₂O) and ascorbic acid. After washing with physiologic saline, labeled platelets were infused into human subjects. Platelet survival and turnover and body surface counting were done. Organ distribution of ^99mTc-labeled platelets was observed by scintillation camera.

Technetium-99m fulfilled all requirements of an ideal cell label. Platelets can be labeled for a brief period of time and there is no evidence that the label damages the platelets or is eluted from platelets under the conditions of the present study.

In 13 out of 16 patients, sequestration observed by body surface counting coincided with sites of sequestration observed using a scintillation camera. In three cases in which platelet destruction rather than sequestration was present, the results using both techniques were different.

It appeared that camera images obtained with ^99mTc-labeled platelets would improve the limited collimation of a small part of the organ obtained by body surface counting and make possible quantitative measurement of the sites of platelet sequestration.