HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Chen, I-W. Articles by Goldsmith, R. E. Search for Related Content PubMed PubMed Citation Articles by Chen, I-W. Articles by Goldsmith, R. E.

Radioimmunoassay of Parathyroid Hormone: Peripheral Plasma Immunoreactive Parathyroid Hormone Response to Ethylenediaminetetraacetate

University of Cincinnati, College of Medicine, Cincinnati, Ohio

Correspondence: For reprints contact: I. W. Chen, Radioisotope Laboratory, Cincinnati General Hospital, Cincinnati, Ohio 45229.

ABSTRACT

Plasma immunoreactive parathyroid hormone (IPTH) concentration was measured by radioimmunoassay according to the modified procedures of Arnaud and coworkers. Thirteen healthy control subjects and 20 patients with operatively proved primary hyperparathyroidism were studied. Each was given an infusion of ethylenediaminetetraacetate (EDTA) to produce a standard hypocalcemic insult. The parameters examined were the basal IPTH value, the peak post-EDTA IPTH value, the difference between these two values (absolute rise), and the ratio of these two values (EDTA-Index). All parameters were found to be significantly different between control and disease groups. Eleven of these 16 were studied postoperatively. In the nine thought to be "cured," the post-operative EDTA studies revealed significant change toward normality. It is concluded that (A) parathyroid tissue in patients with primary hyperparathyroidism is under at least positive feedback control; (B) the hyperfunctioning parathyroid tissue in primary hyperparathyroidism patients is the hyperresponsive tissue; and (C) using our antiserum, the parameters derived from the EDTA study have served to separate normal subjects from patients with primary hyperparathyroidism better than the basal plasma IPTH concentration alone.

FOOTNOTES

^* Present address: Nuclear Medicine Laboratory, Indiana University Medical Center, 1100 W. Michigan St., Indianapolis, Ind. 46202.