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Radiochromate-Binding Capacity of Human Tonsillar Lymphocytes

Institute of Medical Chemistry, Semmelweis University of Medicine, Budapest, Hungary

Correspondence: For reprints contact: F. Antoni, Institute of Medical Chemistry, Semmelweis University of Medicine, Puskin 9, 1088 Budapest, Hungary.

ABSTRACT

The binding of radiochromate to tonsillar lymphocytes, the distribution of label, and the in vitro protein and nucleic acid synthesis of ⁵¹Cr-labeled cells has been investigated.

The uptake of chromate by the cells is influenced by a variety of factors, namely, labeling time and concentration of chromate and of cells. The amount of chromate bound to a single cell increases with increasing cell concentration.

The ⁵¹Cr labeling does not influence the in vitro protein and nucleic acid synthesis of the cells.

It is shown that the distribution of label concerning the different cell fractions is determined by the chromium content of the cells. The labeling of the fraction insoluble in water and NP-40 is relatively high when the fraction is prepared from cells of high chromate content. Proteins produced in the course of in vitro incubation are also found to bind chromate.

The results are discussed concerning the reliability and reproducibility of cytotoxicity assays and the interaction of chromate with lymphocytes.