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Cellular Necrosis Model in Tissue Culture: Uptake of ^99mTc-Tetracycline and the Pertechnetate Ion

Harvard Medical School, Boston, Massachusetts

Correspondence: ¹ For reprints contact: Eileen W. Prince, Joint Program in Nuclear Medicine, 50 Binney St., Boston, Mass. 02115.

ABSTRACT

The recent development of ^99mTc-tetracycline in our laboratory and its successful use in delineating myocardial infarcts have encouraged further investigation into the mechanism of its uptake and its localization on a subcellular level. The affinity of ^99mTc for cells and subcellular constituents when incubated with ^99mTc-tetracycline was evaluated in our recently developed necrosis model of tissue culture. Live cells obtained from exponentially growing cultures or dead cells obtained from the media of nutritionally depleted plateau-phase cultures were incubated with ^99mTc-tetracycline at 37°C for various times. After incubation, the cells were washed and the total cellular activity as well as the sub-cellular localization of ^99mTc determined. About 60 times more ^99mTc remained associated with the dead cells than with the live cells. This affinity was not seen when the dead cells were incubated with the pertechnetate ion. On the sub-cellular level, at least 50% of the ^99mTc appeared to be associated with the DNA and protein fractions of the cell. This system may provide a rapid and economical means of screening radioactive compounds currently being prepared to detect necrotic tissue.