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Peptidase Activity of Carrier Proteins used in Radioimmunoassays

Schwarz/Mann Division of Becton, Dickinson and Company, Orangeburg, New York

Correspondence: ¹ For reprints contact: S. Gutcho, Schwarz/Mann Division of Becton, Dickinson and Co., Orangeburg, N.Y. 10962.

ABSTRACT

Preparations of lysozyme, bovine serum albumin, and human serum albumin may contain levels of peptidases affecting the stability of angiotensin I and ¹²⁵I-angiotensin I (Asp¹.Ile⁵), the standard and tracer in the radioimmunoassay of angiotensin I. Enzyme activity was evaluated by a radioimmunoassay method measuring the loss of immunoreactivity of angiotensin and an autoradiographic method permitting a quantitation and characterization of fragments of angiotensin. Damage ranged from very slight to extensive and was consistent with chymotrypsin or chymotrypsin-like activity. Evaluation of carrier proteins used in radioimmunoassays is essential for long-term stability of peptides.