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Rapid Method for Preparation of Human Fibrinogen Labeled with ¹²⁵I

Veterans Administration Hospital, Albany, New York

Correspondence: For reprints contact: Richard A. Peabody, Research Service, VA Hospital, Albany, N.Y. 12208.

ABSTRACT

A method for the purification of human fibrinogen from fresh frozen plasma which can be completed in less than 5 hr is described. An initial cryoprecipitation step is followed by two selective precipitations of fibrinogen with polyethylene glycol 4,000. The average purity of 62 preparations was 93%. Yields of over 50% of the fibrinogen present in the starting plasma were routinely obtained. The fibrinogen obtained is suitable for labeling with ¹²⁵I. A procedure for labeling fibrinogen, modified from work of others, which makes the isolation and radioactive labeling of fibrinogen complete within one working day is described in this study.