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The Journal of Nuclear Medicine Vol. 15 No. 11 974-980
© 1974 by Society of Nuclear Medicine
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"Kit" Preparation of Radioiodinated Autologous Fibrinogen Using 131I-Monochloride

Philip Hagan*, Michael D. Loberg, Buck A. Rhodes, Katherine Harrison and Malcolm D. Cooper

University of Maryland School of Medicine and School of Pharmacy, and Johns Hopkins Medical Institutions, Baltimore, Maryland

Correspondence: For reprints contact: M. D. Loberg, Div. of Nuclear Medicine, University of Maryland Hospital, 22 S. Greene Baltimore, Md. 21201.

ABSTRACT

A rapid method for the production of radioiodinated autologous fibrinogen (RAF) is given. The method is complete within 1 hr, can be conveniently handled in kit form, produces fibrinogen that is 85–90% clottable, and eliminates the risk of serum hepatitis transmission. Iodination yields of 48–60% were obtained over a range of iodine-to-fibrinogen ratios of 0.5–3.0 using the Helmkamp modification of the iodine monochloride labeling procedure. The physicochemical damage to the fibrinogen molecule owing to the radiolabeling procedure was determined by chromatographing RAF on Bio Gel A 1.5 m and 15 m. The biologic authenticity of RAF was assayed employing both plasma clearance and tissue-distribution studies in dogs. Plasma half-lives of 52–58 hr were found corresponding to the known rate of fibrinogen catabolism in dogs. Three and one-half, 6, and 7.6% of the injected RAF was found in the thyroid, stomach, and liver, respectively, at 24 hr. The results indicate that this rapid method produces biologically active radiolabeled fibrinogen suitable for routine clinical use.

FOOTNOTES

* Present address: VA Hospital, Pharmacy Service, 3350 LaJolla Village Dr., San Diego, Calif.






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Copyright © 1974 by the Society of Nuclear Medicine.