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A Possible Artifact in Gel Chromatography of Some ^99mTc-Chelates

University of Sydney, Royal Prince Alfred Hospital, Sydney, Australia

Correspondence: For reprints contact: Peter E. Valk, Donner Laboratory, University of California, Berkeley, Calif. 94720.

ABSTRACT

Gel chromatography of the renal agent ^99mTc-Sn-gluconate showed two fractions: a chelate fraction and a fraction retained in the column. When this agent was prepared and analyzed on four occasions, there was no significant variation in biological distribution in the rat, but retention of activity in the column varied from 10 to 60%. The biological behavior of the chelate fraction eluted from the column did not differ from that of the unfiltered preparation. The successive passage of the chelate through two columns resulted in retention of less than 5% by the first column and 65% by the second. No difference was found in the biological behavior of the unfiltered, filtered, and refiltered chelate.

The results indicate that the noneluted fraction shown by gel chromatography of this ^99mTc-helate is an artifact produced by the analytical procedure. In studying the chemistry of radio-pharmaceuticals, the results of analytical separations must be correlated with the biological behavior of the separated fractions to establish the validity of the separation procedure.

FOOTNOTES

^* Present address: Donner Laboratory, University of California, Berkeley, Calif. 94720.