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Incorporation and Release of ⁵¹Cr and ¹⁴C-Labeled Amino Acids in Ehrlich Ascites Carcinoma Cells

Roswell Park Memorial Institute, Buffalo, New York

Correspondence: For reprints contact: David E. Maslow, Dept. of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, N.Y. 14203.

ABSTRACT

The uptake, incorporation and release of ¹⁴C-labeled amino acids and ⁵¹Cr were studied in Ehrlich ascites tumor cells. While the rates of uptake of radioactive materials into the cells and lysed cell supernatant were not significantly different, ¹⁴C-amino acids were recovered in greater quantity from the lysed cell centrifugate and the TCA-precipitable material. The nondialysable ¹⁴C-labeled material also had higher activity than that labeled with ⁵¹Cr. The ⁵¹Cr- and ¹⁴C-labeled materials were released from cells over a range of osmolalities in a pattern parallel to that of cell death as measured by trypan blue exclusion. Cellular exudation of ⁵¹Cr-labeled material and ¹⁴C-amino acid-labeled material, associated with cell adhesion, were similar and increased with time. The results confirm the usefulness of ⁵¹Cr labeling as an accurate marker of cytolysis and cell exudation.