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A "Kit" Method for the Preparation of a Technetium-Tin(II) Colloid and a Study of Its Properties

Donner Laboratory, University of California at Berkeley, Berkeley, California

Correspondence: For reprints contact: Max S. Lin, Division of Endocrinology and Nuclear Medicine, Michael Reese Hospital and Medical Center, 29th St. and Ellis Ave., Chicago, Ill., 60616.

ABSTRACT

A "kit" method for the preparation of a technetium-tin(II) colloid was presented. The entire procedure for preparing the colloid consisted of adding technetium generator eluate and albumin solution in that order to an aqueous tin(II) in a vial.

The aqueous tin(II) was made in water in a standardized fashion. The sterility of this aqueous tin(II) and its preservation in a "kit" form were accomplished by Millipore filtering the aqueous tin(II) into sterile vials and subsequent purging of the air inside the vial with Millipore-filtered nitrogen. When kept at 4°C under the nitrogen, the aqueous Sn(II) was stable for at least 20 days.

Ionic aluminum(III) added to the generator eluate to a concentration up to 10 µg Al/ml colloid did not have adverse effect on the quality of the coilloid. The aluminum at concentrations up to 20 µg Al/ml colloid failed to induce flocculation in the colloid when evaluated by inspection and by in vivo assay.

The colloid was reproducible. Its free technetium content was in the order of 0.1%. Ten minutes after its intravenous administration, the technetium uptake in the liver plus spleen was 97% with a range of 93–102% using 25 batches of the colloid in 130 rats. The technetium was not permanently retained by these reticuloendothelial organs. By 51 hr, a little over one third of the initial technetium uptake in these organs had been released and excreted.

The colloid was stable in standing either under nitrogen or under air for over 10 hr. It was also stable against agitation that would not cause foaming in the colloid.

In terms of the simplicity of its preparation procedure, its relative insensitivity to contaminating ionic aluminum(III), its low content of the technetium below and above the colloidal range, and the rate of release and excretion of the technetium following its initial localization in the liver and the spleen, the present technetium-tin(II) colloid appeared to compare favorably with technetium-sulfur colloids.