HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tran, N. Articles by Lebel, E. Search for Related Content PubMed PubMed Citation Articles by Tran, N. Articles by Lebel, E.

Oxidation of DL-3-Phenylalanine-1-¹⁴C, DL-Leucine-1-¹⁴C, and D-Glucose-1-¹⁴C-6-Phosphate to ¹⁴CO₂ in Human Placenta

Centre Hospitalier Universitaire, Sherbrooke, Québec, Canada

Correspondence: For reprints contact: Ngo Tran, Dept. of Nuclear Medicine and Radiobiology, Centre Hospitalier Universitaire, Sherbrooke, Québec, Canada.

ABSTRACT

An ionization chamber method was used for instantaneous and continuous measurement of ¹⁴CO₂ production from DL-3-phenylalanine-1-¹⁴C, DL-leucine-1-¹⁴C, and D-glucose-1-¹⁴C-6-phosphate in fresh human placenta. The results obtained demonstrated the presence of phenylalanine hydroxylase, -ketoacid oxidases, and glucose-6-phosphate dehydrogenase involved in the oxidation of these labeled substrates in the placenta, respectively. This study offers an in vitro method for early detection of normal homozygotes, heterozygotes, and atypical homozygotes of hyperphenylalaninemia, maple syrup disease, and glucose-6-phosphate dehydrogenase deficiency in the newborn infants without any radiation danger.