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Estimation of the T₄ Binding Capacity of Serum TBG and TBPA by a Single T₄ Load Ion Exchange Resin Method^*

Thyroid Function Laboratory, University of Chicago, Pritzker School of Medicine, Chicago, Illinois
Thyroid Laboratory, Peter Bent Brigham Hospital, Boston, Massachusetts

Correspondence: For reprints contact: Samuel Refetoff, Thyroid Study Unit, University of Chicago, 950 E. 59th St., Chicago, Ill. 60637.

ABSTRACT

The principle and method of a simple assay for the determination of the thyroxine (T₄)-binding capacity of thyroxine binding globulin (TBG) and thyroxine binding prealbumin (TBPA) are described. The procedure is accurate, reproducible, rapid, and has been adopted for routine hospital use. A single technician can perform in a regular working day 60 determinations in addition to the three internal control samples. Results obtained using this method compared favorably with those obtained electrophoretically and by radioimmunoassay. Mean and standard deviations for the TBG capacity in the normal adult population are 19.2 ± 4.0 in males, 21.6 ± 4.0 in females, 44.1 ± 5.9 in patients taking estrogen containing medications, 53.7 ± 7.1 in the last trimester of pregnancy, 14.1 ± 3.1 in patients with renal failure, 15.7 ± 3.2 in hyperthyroid, and 24.6 ± 5.1 µg/100 ml in hypothyroid patients. These results are in agreement with previously reported values using different assay methods. Data obtained from five families with X-chromosome linked inherited TBG abnormalities suggest that the defect is quantitative rather than due to a protein varying in its ability to bind thyroxine.

FOOTNOTES

^* Comparison of two unrelated methods, survey of population, and abnormalities in various diseases.

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