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Determination of Specific Activity of ³²P-Labeled Compounds Using Cerenkov Counting

Beth Israel Hospital and Harvard Medical School, Boston, Massachusetts

Correspondence: For reprints contact: Peter B. Schneider, Beth Israel Hospital, 330 Brookline Ave., Boston, Mass. 02215.

ABSTRACT

Specific activity of ³²P-labeled compounds can be conveniently measured by using Cerenkov counting of the same sample used for the chemical determination of phosphorus by colorimetry. Variable color quenching is eliminated by bleaching the sample prior to Cerenkov counting. The method is particularly suitable for the analysis of samples separated by thin-layer chromatography.