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The Ashing Technique as a More Precise Method for the Radioassay of ⁵¹Cr in Feces

University of Michigan, Ann Arbor, Michigan

Correspondence: For reprints contact: Donald H. Kuiper, Dept. of Medicine, College of Human Medicine, Michigan State University, East Lansing, Mich. 48823.

ABSTRACT

Two methods for the radioassay of stool for blood loss using ⁵¹Cr tagged erythrocytes were compared. One method assayed a sample of the 24-hr stool homogenate, while the other method assayed the ash of the entire 24-hr fecal collection. The ashing technique revealed better precision in recovery as shown by a s.d. of 3.0 as compared to a s.d. of 32.0 using the wet technique. The ashing method concentrates a large volume of stool, thereby yielding higher counts per sample and thus reducing the error in counting.

FOOTNOTES

^* See present address above.