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Incorporation of ⁷⁵Se-Selenomethionine in Human Peripheral Lymphocytes as an In vitro Test of Globulin-Synthesizing Capacity

University of Iowa, Iowa City, Iowa

Correspondence: For reprints contact: F. H. F. Cheng, Nuclear Medicine, University of Iowa Hospitals, Iowa City, Iowa 52240.

ABSTRACT

An in vitro assay procedure has been described for measuring the globulin-synthesizing capacity of human peripheral lymphocytes in the presence of ⁷⁵Se-selenomethionine and phytohemagglutinin (PHA). Lymphocytes cultured at 37°C for 24 hr incorporated small amounts of ⁷⁵Se-selenomethionine into globulin protein in unstimulated cultures, while lymphocytes in the presence of PHA incorporated a much greater amount of this amino acid analog in stimulated cultures. The ratio between radioactivities of stimulated cultures and unstimulated cultures gave an index of stimulation by PHA on the globulin synthesizing capacity of lymphocytes. Values of such an index of stimulation by PHA for lymphocyte cultures obtained from twenty clinically normal individuals were 2.75 ± 0.63. Several parameters and reproducibility of the assay method were also tested.