FIGURE 6. Colon radioactivity uptake is decreased after anti-4 antibody injection. SPECT was performed on mice with TNBS colitis 48 h after transfer of ¹¹¹In-labeled TNBS-sensitized lymphocytes and injection of control antibody or anti-4 antibody. (A) CUR was calculated normalized to bone marrow uptake in pelvis. Box plots of mean radioactivity uptake of 2 groups are indicated (*P = 0.012 [statistically significant]). (B) Isolated colons were also counted in -counter; -counter results were corrected for decay and expressed as cpm/g tissue. To correct for nonspecific binding, ratios were calculated normalized to radioactivity in blood: (cpm/g colon – cpm/g blood)/(cpm/g blood) (*P = 0.05 [statistically significant]).