FIGURE 3. Effect of estrogen depletion on cAT₁ receptor expression in adrenal gland as determined by PET imaging in vivo. (A) K_i changes in individual animals first off and then on E₂ (off-on model) and in animals first on and then off E₂ (on-off model). (B) K_i in 2 groups of animals (off-on and on-off model) and in all pooled animals off and on E₂. As in kidney (Fig. 2), differences between off and on E₂ were consistent but statistically not significant in separate off-on and on-off subgroups. Differences between pooled parameters were statistically significant (P = 0.003).