FIGURE 2. Specificity of binding reactivity of sera from mice immunized with different DOTA carriers. Binding activity was assessed on biotinylated HSA (hatched bars) or cM19 (solid bars), conjugated (A) or not conjugated (B) to DOTA, by quantitative ELISA. Sera (diluted 1:200 and tested in duplicate) were obtained after third injection (day 35) of immunogens, as described in Table 2. Data are mean ± SD of 2 replicates. A = absorbance.